A colorimetric and fluorescent probe for detecting intracellular GSH Ondřej Zítka Datum konání: 4.12.2015 Investice do rozvoje vzdělávání
Glutathion (GSH) Tripeptide Glutathion has many biological functions Glutathione exists in both reduced (GSH) and oxidized (GSSG) states. In the reduced state, the thiol group of cysteine is able to donate a reducing equivalent (H++ e−) to other unstable molecules, such as reactive oxygen species.
The probe Scheme 1. The design concept of probe 1 for GSH.
The parameters of the probe
The specifity
The NMR spectra
The cell test
Summary New rapid and highly sensitive coumarin-based probe (probe 1) has been designed and synthesized for detecting intracellular GSH It was converted into a latent fluorescence probe, which allowed it to achieve high sensitivity (LOD 122 nM) and fluorescence turn-on response (F/F0>15) toward GSH over other various natural amino acids in PBS buffer solution at pH 7.4.
Investice do rozvoje vzdělávání Acknowledgements: Project: Paper: Budování výzkumných týmů a rozvoj univerzitního vzdělávání výzkumných odborníků pro mikro- a nanotechnologie (NANOTEAM) CZ.1.07/2.3/.00/09.0224 Chen C, Liu W, Xu C, Liu W (2015) A colorimetric and fluorescent probe for detecting intracellular GSH. Biosensors & Bioelectronics 71: 68-74 Investice do rozvoje vzdělávání