Budování výzkumných týmů a rozvoj

Prezentace na téma: "Budování výzkumných týmů a rozvoj"— Transkript prezentace:

1 Budování výzkumných týmů a rozvoj
CZ.1.07/2.3/.00/ NANOTEAM Budování výzkumných týmů a rozvoj univerzitního vzdělávání výzkumných odborníků pro mikro- a nanotechnologie Seminář na téma: Laboratory of biomarkers and flow techniques Ondřej Zítka Ústav chemie a biochemie, místnost D06

2 Members of laboratory RNDr. Ondřej Zítka, Ph.D.
Mgr. Natálie Cernei, Ph.D. Ing. Jiří Kudr Mgr. Roman guráň Mgr. Markéta Komínková Bc. Zuzana Lacková Bc. Michal Pláteník (technology part) Jan Zítka (technology part)

3 Activities of laboratory
Servis analysis Total aminoacids determination Thiol compounds determination GSH/GSSG, Phytochelatins Isolation of protein using FPLC MALDI identification of proteins MALDI identification of bacteria species Research activities: Developement of analytical methods for study of low molecular metabolites MALDI imaging methods Design and building of Flow injection analysis methods and instruments for isolation and detection of biomolecules using PMPs Design and manufacturing of technology devices

4 Major research activities
Low molecular metabolites– Antioxidants, thiolic peptides, Fytochelatin synthase EC HPLC with electrochemical detection Zitka, O., Krystofova, O., Sobrova, P., et al, (2011c) Phytochelatin synthase activity as a marker of metal pollution. J. Hazard. Mater., 192,

5 Major research activities
Low molecular metabolites– Aminoacids, Sarcosine, (Glutamine) Currently analysis for LPR project Ion exchange liquid chromatography

6 Major research activities
Low molecular metabolites– SAM, SAH - potential markers in cancer diagnostics HPLC-MS (Q-TOF) Chromatogram 2 4 6 8 10 Time [min] 1 x10 Intens. SAH,SAM kalibrace_0.004_2.d: EIC All MS SAH,SAM kalibrace_0.004_2.d: EIC All MS SAH,SAM kalibrace_0.0004_2.d: EIC All MS SAH,SAM kalibrace_0.0004_2.d: EIC All MS SAH,SAM kalibrace_ _3.d: EIC All MS SAH,SAM kalibrace_ _3.d: EIC All MS Spectrum View SAH,SAM kalibrace_0.004_2.d: +MS, 4.6min #278 100 200 300 400 500 600 700 m/z SAH,SAM kalibrace_0.004_2.d: +MS, 2.8min #167 5 800 900 LOD 0,06 µM LOQ 0,21 LOD 0,14 µM LOQ 0,48

7 Major research activities
Proteins – MALDI imaging – under the method developement MALDI – TOF/TOF

8 Major technology /research activities
Low molecular metabolites – Flow injection analysis with paramagnetic particles Building and construction of new fluidic devices Syringe Pump waste Switching valve Detector (CHI Cell) (control) Sample/rinsing buffer inlet PC Dynabeads Protein G Goat Imunoglobulins Mouse Imunoglobulins 8-hydroxyguanosin Step 1: Imunocomplex G Step 2: Imunocomplex G-M Step 3: Imunocomplex G-M-R + Rabbit Anti-mouseImunoglobulins Modified by Alcaline phosphatase Zitka, O., Krizkova, S., Krejcova, L., et al (2011b) Microfluidic tool based on the antibody-modified paramagnetic particles for detection of 8-hydroxy-2´-deoxyguanosine in urine of prostate cancer patients. Electrophoresis, 32,

9 Major technology /research activities
Dosing machine for screen printed electrodes Real device 3-D Model Controlr unit Electrode reservoir waste Sample imput Cable to potentiostat 3D printing technology

10 Major technology /research activities
Design and manufacturing of Flow injection analysis device for mixing using clever magnetic field coupled with electrochemical detection – technology project Core of electrochemical cell (PDMS) Placement of electromagnetic stirrer Housing of core (ABS). Cell inputs Upper output for preasure regulation in the cell output Cell volume 2000 (ul)

11 Major technology /research activities
Process of manufacturring of prototype… Core of electrochemical cell (PDMS) Positive template (ABS) for negative template from low melting point metal (melting point 75°C) Housing of core (ABS)

12 Major technology /research activities
Process of manufacturring of prototype… Negative template (Wood´s metal - Bi/Pb/Cd/Sn) Final product (PDMS)

13 Reconstruction of technology part
Plan of laboratory of technology after reconstruction EXP. stůl EXP 2 EXP 1 EXP 4 EXP 5 EXP 3 Mechanické úpravy Mechanické obrábění soustruh Mechanické obrábění fréza Návrhy konstrukci a programovaní + pracovní místo Pájecí stanice A testování Osazování pcb optika Pracovní místo u exp stolu + záloha 3D tiskárna Bruska Ovládání 3D tiskárny + pracovní místo (Vstup) (Vstup)

14 …to all colleagues from the Department of chemistry and biochemistry
Acknowledgements …to all colleagues from the Laboratory of biomarkers and flow techniques …to all colleagues from the Department of chemistry and biochemistry